USO DA CITOMETRIA DE FLUXO COMO UMA METODOLOGIA EMERGENTE PARA AVALIAR A VIABILIDADE CELULAR PROBIÓTICA
Resumo
A quantificação bacteriana em uma formulação probiótica de forma precisa é imprescindível para garantir que o produto atingiu a dose mínima de células recomendada, cumprindo assim, os padrões regulamentares e a citação no rótulo. Métodos tradicionais de contagem em placas não enumeram com exatidão todos os probióticos disponíveis, apenas a população bacteriana capaz de se replicar sob em meios de cultura em circunstâncias específicas. Portanto, não contabilizam as bactérias que sob condições ambientais estressantes tornam-se não cultiváveis, embora se mantenham metabolicamente ativas e possam retornar a capacidade de se replicar quando as condições se tornarem favoráveis novamente. Por outro lado, metodologias independentes de cultura, como a citometria de fluxo, possui uma definição mais abrangente e confiável da viabilidade bacteriana por incluir as bactérias viáveis mas não cultiváveis (VNC), discriminando subpolulações celulares vivas, danificadas e mortas com precisão e rapidez, facilitando assim, a rotina de laboratórios. Nesta revisão são abordados aspectos gerais da citometria de fluxo, bem como as principais vantagens da utilização para avaliação detalhada da viabilidade e funções fisiológicas celulares quando comparado às técnicas tradicionais de cultura.Referências
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